After collecting virus sample, usually the sample can not be detected in time, it is necessary to use Viral Transport Medium, for different detection purposes, different Viral Transport Medium need to be used, and in the different virus detection experimental conditions, it is divided into inactivated Viral Transport Medium andcultured Viral Transport Medium.
Inactivated Viral Transport Medium contain highly effective viral lysate, usually using guanidine salts or other effective viral inactivators, which rapidly inactivate the virus after sampling and release viral nucleic acids for easy detection. At the same time, the inactivated Viral Transport Medium can protect the collector well, which is safe and reliable for the collector and the transportation process, and effectively prevents the risk of secondary infection.
The Cultured Viral Transport Medium does not contain lysate, but a variety of solution components that are conducive to the culture of viral host cells, ensuring the integrity of the virus and helping to increase the survival time and infection stability of the virus. The sample provided by Cultured Viral Transport Medium is basically a live virus, which can maintain the originality of the sample to the greatest extent, and can be used not only for nucleic acid extraction and detection of the virus, but also for the culture and isolation of the virus. However, when used for detection, it should be noted that nucleic acid extraction and purification must be performed after inactivation.
Advantages of inactivated Viral Transport Medium