The difference between Rapid Antigen Test detection and nucleic acid detection The basic principles of nucleic acid detection and Rapid Antigen Test detection are different: Nucleic acid detection is to check the RNA of the virus in the sample, while Rapid Antigen Test detection is to measure the quantity of the N protein of the new coronavirus. Rapid Antigen Test detection results are much shorter than nucleic acid detection, the detection time is about 10-15min. How to standardize the operation of Rapid Antigen Test detection Preparation before Rapid Antigen Test Wash hands with running water or hand sanitizer. Carefully read the instruction manual of the Rapid Antigen Test reagent and the precautions related to the Rapid Antigen Test. Check whether the Rapid Antigen Test reagent is within the shelf life, and check whether the contents of the nasal swab, sampling tube, test card wheather are missing or damaged. If the reagent expires or the contents of the reagent are missing or damaged, the detection reagent should be replaced in time. Confirm the testing requirements for ambient temperature and humidity. The detection of colloidal gold test strips generally requires a temperature of 14°C-30°C to avoid abnormal test results caused by excessively cold, overheated or excessively humid environments. Sample collection When taking nasal swab sampling, tilt your head slightly and go 1-1.5 cm deep into the bottom of the nostril. If the operation method is incorrect, it may affect the sampling of the sample and the accuracy of the test. Sample detection If both the C line and the T line show a red band, the result is positive; If the C line has a red band and the T line does not, the result is negative There is no red band at the C line, indicating that the test result is inaccurate, and the test should be re-tested.

qPCR Rt detection of Pcr Nasal Swab (N) and PCR throat swab (T) samples is the gold standard for the diagnosis of sars cov 2. What is the difference and connection between the two swab specimen collection method: Pcr Nasal Swab and PCR throat swab Specimen Collection? Both Pcr Nasal Swab and PCR throat swab are made through nylon flock technique. The fluff on the flocking Swab Specimen Collection area can increase the sampling surface of the qPCR Rt  swab in contact with the physical evidence and improve the accuracy of the qPCR Rt test compared with traditional cotton swab. According to relevant researches, for the qPCR Rt test results of the specimen taken from the same patient by both Pcr Nasal Swab and PCR throat swab, it was found that the positive rate of Pcr Nasal Swab specimen was higher than that of PCR throat swab specimen, that is, Pcr Nasal Swab Specimen was more sensitive. The sampling efficiency of Pcr Nasal Swab than that of PCR throat swab. The study found that the virus amount in the nose of patients with respiratory virus infection may be more than that in the pharynx. In the clinical practice of swab Specimen Collection, when healthcare provider collect PCR throat swab specimen, taking into account the transmission routes of sars cov 2 in the form of droplets and aerosols, the sampling time is often short and rough, which will affect the accuracy of the results. When collection Pcr Nasal Swab specimen, the healthcare provideris on the side of the patient, which can reduce the infection risk of sars cov 2. In conclusion, compared with PCR throat swab, Pcr Nasal Swab have obvious advantages in qPCR Rt test swab Specimen Collection.

Protection Of Specimen Collection Personnel During the Pcr Nasal Swab Test, the specimen collectors shall be equipped with protective equipment according to the three-level protection, including work clothes, protective clothing, disposable work caps, medical protective masks (N95), surgical masks, goggles, masks, double-layer gloves, waterproof boots and shoe covers. Equip facilities to prevent the spread and infection of pathogenic microorganisms, such as garbage cans for infectious waste disposal, medical tools for handling emergencies, and certain ventilation conditions. Pcr Nasal Swab Test Specimen Collection Procedure Use Nylon Flocked Nasopharyngeal Swab to collect the nasopharyngeal samples. Measure the distance from the tip of the nose to the earlobe with a Nylon Flocked Nasopharyngeal Swab and mark it with your finger. Insert the Nylon Flocked Nasopharyngeal Swab into the nasal cavity in a direction perpendicular to the nose (face). The Nylon Flocked Nasopharyngeal Swab should be at least half the length from the earlobe to the tip of the nose. Keep the Nylon Flocked Nasopharyngeal Swab in the nose for 15 to 30 seconds. Rotate it gently for 3 to 5 times. Quickly put the Nylon Flocked Nasopharyngeal Swab into a sample collection tube containing viral transport medium containing RNA enzyme inhibitors. After inserting the Nylon Flocked Nasopharyngeal Swab, break theNylon Flocked Nasopharyngeal Swabrod near the top, tighten the tube cap and seal it with a sealing film.