After collecting virus sample, usually the sample can not be detected in time, it is necessary to use Viral Transport Medium, for different detection purposes, different Viral Transport Medium need to be used, and in the different virus detection experimental conditions, it is divided into inactivated Viral Transport Medium andcultured Viral Transport Medium. Inactivated Viral Transport Medium Inactivated Viral Transport Medium contain highly effective viral lysate, usually using guanidine salts or other effective viral inactivators, which rapidly inactivate the virus after sampling and release viral nucleic acids for easy detection. At the same time, the inactivated Viral Transport Medium can protect the collector well, which is safe and reliable for the collector and the transportation process, and effectively prevents the risk of secondary infection. Cultured Viral Transport Medium The Cultured Viral Transport Medium does not contain lysate, but a variety of solution components that are conducive to the culture of viral host cells, ensuring the integrity of the virus and helping to increase the survival time and infection stability of the virus. The sample provided by Cultured Viral Transport Medium is basically a live virus, which can maintain the originality of the sample to the greatest extent, and can be used not only for nucleic acid extraction and detection of the virus, but also for the culture and isolation of the virus. However, when used for detection, it should be noted that nucleic acid extraction and purification must be performed after inactivation. Advantages of inactivated Viral Transport Medium Safer: Inactivated Viral Transport Medium contains a variety of virus inactivation components, which effectively inactivates viruses and ensures the safety of transportation and testing personnel. More stable: The Viral Transport Medium contains RNase inhibitory components to ensure the integrity of nucleic acids. Eliminate false negative results. More convenient: Supporting flocking swab, reasonable design, not only increases the safety of the operator but also ensures stable adsorption and release of samples, compared with traditional cotton swab sampling, the same operation can collect more samples at one time.

Collection Procedure of Nylon Flocked Nasopharyngeal Swab Instruct the patient to remove the mask and remove the secretions at the front of the nasal cavity; Open the Nylon Flocked Nasopharyngeal Swab package. Instruct the patient to tilt his head gently back to make it easier for the Nylon Flocked Nasopharyngeal Swab to pass through the nasal passage. Gently insert the Nylon Flocked Nasopharyngeal Swab vertically into one nostril and slowly push the Nylon Flocked Nasopharyngeal Swab forward along the nasal septum until resistance is felt. If it is difficult to push, exit and adjust the angle before pushing. Leave the Nylon Flocked Nasopharyngeal Swab standing for a few seconds (or rotate it in place for several times) to fully absorb the mucus, and then slowly return the Nylon Flocked Nasopharyngeal Swab while rotating the handle. Ask the patient to put on the face mask again. How can I tell if a Nylon Flocked Nasopharyngeal Swab has actually probed the posterior pharyngeal wall? Estimate Depth: Before opening the Nylon Flocked Nasopharyngeal Swab package, estimate the distance between the nostril and the anterior end of the auricle as the depth of the Nylon Flocked Nasopharyngeal Swab entering the nasal passage when it touches the posterior pharyngeal wall. According to this depth, traces can be left in the corresponding position on the Nylon Flocked Nasopharyngeal Swab handle in advance, which is convenient for reference during operation. When resistance is felt in advance: If you feel resistance when pushing the Nylon Flocked Nasopharyngeal Swab before reaching the estimated depth, you can retreat slightly, change the angle slightly to bring the Nylon Flocked Nasopharyngeal Swab closer to the lower wall of the nasal passage, and try to push forward again. Reference - CDC guidance on specimen collection for COVID-19 testing (last updated on 2020.04.29) Link: https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html

Nucleic Acid Testing Antigen Detection Detection of samples Nasopharyngeal nylon flocked swab or oropharyngeal nylon flocked swab, sputum nasopharyngeal nylon flocked swab or anterior nasal nylon flocked swab, Sputum or pulmonary Detection target Genes: RdRp, Orflab, and E 、N 、S Structural proteins : S, N, M, and E Detection window period Early stage of infection, until eliminate the virus after recovery Early stage of infection, until eliminate the virus after recovery Detection Time 2-4 Hours Within 15 minutes Detection requirement At least BSL-2 laboratory No special requirements Advantages & Disadvantages Advantages: High sensitivity,high specificity ; Disadvantages: Time-consuming and labor intensive Advantages: easy operation, rapid test time, low cost, the environmental requirements are not high ; Disadvantages: Samples with low levels of virus may not be detected. It is best to use nucleic acid detection methods to check and confirm to avoid misjudgment.